जैव रसायन और आण्विक जीवविज्ञान जर्नल खुला एक्सेस

अमूर्त

Deregulation of Plasma MiR-21 for Early Detection of Lung Cancer among Palestinian People Exposed to High Intensity of Diesel Exhaust Emissions

Ahmed Isam Slaileh, Ashraf Sawafta, Raed Alkowni

For many years, intensive research on the early detection of lung cancer has been of interest to many scientists. Many studies recently focused on using microRNAs as an effective plasma biomarker for the diagnosis of different types of cancer including lung cancer. MIR-21 deregulation was observed in lung cancer patients and plays an important role in tumor development. In this present study, we investigated the plasma miR-21 biomarker as a non-invasive and cost-effective test for early detection of lung cancer among people working at a transportation center in Palestine since these people are exposed to a high level of Diesel Motor Emission (DME). Real time PCR revealed a significant increasing in the expression level of miR-21 in human plasma samples exposed to DME and in Non Small Cell Lung Cancer (NSCLC), than that of normal samples. Moreover, our results indicate a significant correlation between the quantity of plasma miR-21 and the exposure duration to DME (p˂0.05). These results confirm the significance of using miR-21 as a biomarker for the early detection of lung cancer among high risk people.