प्रायोगिक जीवविज्ञान के यूरोपीय जर्नल खुला एक्सेस

अमूर्त

Perturbation of the hypothalamic-pituitary-ovarian axis in experimentally inflicted third degree burns in Wister rats

ADETEYE Olawale Victor and YAMA Oshiozokhai Eboetse

Burns injury categorized by its severity helps determine the pattern of emergency care management. The pathogenesis and mechanisms through which burn trauma interferes with the female endocrine-equilibrium are still poorly understood. To determine the variations in sex hormone in female rats with third degree burns injury. A total of fifteen adult female regularly cycling Wister rats weighing between 150‒200 g were used in the experiments. They were divided into three groups (n=5 rats/ group) designated control, sham, and experimental groups. The control rats were normal usual rats. Third degree burn (TDB) injuries equivalent to 30 % of their total body surface area (TBSA) were inflicted on the skins of the experimental rats. The sham group had 30% of the TBSA shaved with warm water (at room temperature) applied without causing any injury. The rats were sacrificed by cervical dislocation. Blood samples obtained into EDTA specimen bottles. The hormones: Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Prolactin (PRL) and Estradiol (E2) were assayed using the Enzymelinked immunosorbent assay (ELISA) kits. The experimental had a significant increase (p < 0.05) in serum E2 and PRL levels compared to sham and control rats. In contrast however, the mean serum FSH and LH levels of experimental were significantly reduced (p < 0.05). In the Wister rats inflicted TDB there may have been an alteration in the integrity of hypothalamic-pituitaryovarian axis that is responsible for the production of these hormones.