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अमूर्त

Phytochemical and Antimicrobial Evaluation of the Essential Oils and Antioxidant Activity of Aqueous Extracts from Flower and Stem of Sinapis arvensis L

Javad Sharifi Rad, Mahsan Hoseini Alfatemi, Majid Sharifi Rad and Dhrubo Jyoti Sen

The present study has been designed to determine phytochemical and antimicrobial evaluation of the essential oils and antioxidant activity of aqueous extracts from flower and stem of Sinapis arvensis L. (Brassicaceae). The essential oils of plants had a pallid yellowish color with a distinctive sulfury odor. GC/MS analysis of the essential oils exposed a complex mixture of compounds involving monoterpenes, sesquiterpenes, nitriles aldehydes and sulfurcontaining compounds. the essential oil stem of S.arvensis included Benzyl isothiocyante (15.15%), Cubenol (15.12%), dimethyl trisulfide (6.12%), 6,10,14-trimethylpentadecane-2-one (3.85%), indole (1.91%), dimethyl tetrasulfide (2.22%), 1-butenyl isoithiocyanate (18.4%), Thymol (3.44%), octadecane (4.14%), Spathulenol (2.64%), Hexadecane (4.09%), 1-epi–Cubenol (2.41%) and Octadecanal (1.14%) as major constituents. The flower of S.arvensis contained Dimethyl trisulfide (5.24%), Dimethyl trisulfide (19.2%), Dimethyl trisulfide (4.03%), Thymol (4.62%), Indole (1.41%), 2-Phyenyl isothiocyanate (7.45%), δ-Cadinene (3.40%), Spathulenol (1.58%), 9-Methylthiononanonitrile (3.21%), Hexadecane (3.02%), 1-epi–Cubenol (3.01%), Cubenol (14.32%), Octadecane (2.41%), 6,10,14-Trimethylpentadecane-2-one (3.25%), Nanodecane (1.01%) and Octadecanal (1.42%) as major components. In this study, the antibacterial activity of stem and flower essential oils from S.arvensis was evaluated on five pathogenic multi-drug resistant bacteria strains by agar disc diffusion method. The most susceptible Gram-positive and Gramnegative bacteria to the stem and flower essential oils Sinapis arvensis were Staphylococcus aureus (NCTC7428) and P.aeruginosa (MTCC 2453), respectively. According to antioxidant activity resulted, it was highlighted that antioxidant activity of aqueous extract of flower was weaker than stem.

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