बाल रोग एवं स्वास्थ्य अनुसंधान खुला एक्सेस

अमूर्त

Serological Proteome Analysis for Identification of Potential Antigen in Atopic Dermatitis

Blendi Ura, Luigina De Leo, Giorgio Arrigoni, Federica Scrimin, Irene Berti and Tarcisio Not

Objective: Identify human skin derived auto antigens that could be recognized by IgE serum of patients with both severe atopic dermatitis and high serum IgE immunoglobulins.

Design: The study was performed retrospectively on sera collected from children with severe atopic dermatitis with total IgE serum concentration (IgE>3000 U.I./ml), as a serum sample controls we used the children’s sera suffering from severe food allergy and with high IgE concentrations against food proteins (IgE>90 kU/l). All these serum samples were used to identify skin-derived antigen by using immunoproteome technique.

Setting: Institute for Maternal and Child Health.

Patients: Six children suffering from severe atopic dermatitis and six suffering from severe food allergy without skin involvement.

Interventions: Serological proteome analysis (SERPA) for antigen identification.

Main outcome measures: First immunoproteomic study for identification of potential antigen in severe atopic dermatitis. Identification of two immunoreactive proteins IGKC (Ig kappa chain C region) and IGLC1 (Ig lambda-1 chain C regions).

Results: After membranes analysis we identified two immunoreactive proteins IGKC and IGLC1 present only in membrane treated with severe atopic dermatitis. Reaction of these proteins were considered non-specific event and didn’t further investigate.

Conclusions: Using SERPA analysis we identified two immuno-reactive proteins. Even these proteins have proved to be non-specific event; this showed the great potential of SERPA for antigen identification.