Ravi Kant UPADHYAY, Neeraj YADAV and Shoeb AHMAD
In the present investigation toxicity of different solvent extracts of Cassia alata fruits was tested on adults of Callosobruchus chinensis and toxic effects were enumerated on certain biomolecules at an interval of 4hr, 8hr, 12hr and 16hr following sub-lethal exposures. Six different solvent extracts acetone, chloroform, petroleum ether, methanol, hexane and water were prepared and their LD50 values were determined as 0.840μg/gm, 0.510μg/gm, 0.590μg/gm, 1.57μg/gm 0.310 μg/gm and 2.50μg/gm respectively. After exposure of acetone, chloroform, petroleum ether, methanol hexane and water extracts the glycogen content was found to be significantly depleted 39.71%, 37.94%, 41.52%, 33.00%, 45.35%, 44.55&%. Similarly, it cut the protein level up to 49.97%, 49.48%, 58.02%, 49.84%, 58.42% and 68.50%. It has shown depletions in DNA level, 58.95%, 43.44%, 46.85%, 47.97%, 50.86% and 53.38%. RNA content was also found to be reduced51.65%, 62.02%, 67.77%, 63.10%, 58.51% and 82.13%. A significant (p<0.05) depletion in amino acid content was recorded8.81%, 59.03%, 59.58%, 58.59%, 71.10% and 81.09% in comparison to control. However, a slight decrease (74.67%, 58.09%, 74.92%, 66.50%, 58.17% and 58.33%) in lipid level heave also been reported. Almost all extract have act potentially against enzyme, but hexane extract has performed more efficiently and displayed inhibition in ACP (58.52%), ALP (75.39%), GPT (71.86%,), GOT (79.52%), LDH (86.90%,) and AChE (71.65%). Contrarily, aqueous extract have shown lower activity against both bio-molecule and enzymatic parameters, while acetone, chloroform, petroleum ether and methanol have also shown inhibitory activity against these bio-molecules and metabolic enzymes having moderate activity. Hexane extract has shown higher lethality that indicate that it may have more active compounds
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